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A construct was designed to insert an internal ribosome entry site (IRES), cre recombinase gene, Frt site, RNA pol II-Neo-HSVtk-polyA cassette, and a second Frt site downstream of the progesterone receptor (Pgr) transcriptonal stop codon. The construct was electroporated into 129S/SvEv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric males were bred with C57BL/6J females to establish the colony. At some point, mutant mice were bred with GtROSA-FLPe mice (undisclosed genetic background) to remove the frt-flanked neo cassette. (J:196278)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
