Exon 11 was floxed. Cre-mediated recombination removed exon 11 and an FRT-flanked neo cassette inserted downstream of exon 11. Western blot analysis confirmed the absence of PKC-zeta and the isoform protein kinase M-zeta (PKM-zeta) in the brain. Protein expression of other PCK isoforms is normal. (J:194557)