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EN
A cDNA construct encoding EGFP, cre recombinase, human diphtheria toxin receptor (DTR; HBEGF) and click beetle green 99 (CBG99) luciferase was generated, with coding sequences separated by self-cleaving 2A viral sequences. The presence of 2A sequences results in stoichiometric translation of the individual proteins. This construct was inserted at the ATG start codon of Itgax in the RPCI-24-7812 bacterial artificial chromosome by recombineering in bacterial cells. The modified BAC was linearized and injected into pronuclei of fertilized C57BL/6N oocytes and line 2 was established. (J:194800)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
