Tg(Mchr1-cre)1Emf

A cre recombinase sequence (1032 bp) was inserted downstream of the Mchr1 promoter on the RP23-202N16 BAC via homologous recombination/BAC recombineering; this replaced nucleotides 4-41 from exon 1 and was designed to disable transcription/translation of the Mchr1 protein from the BAC Mchr1 locus. An frt-flanked kanamycin selection cassette used during homologous recombination/BAC recombineering was removed by transient FLP plasmid injection. The resulting ~198 kbp Mchr1-cre BAC transgene was injected into C57BL/6J embryos to establish transgenic founder animals. Founder animals were bred to C57BL/6J mice to generate independent founder lines. One founder line was chosen. (J:194588)