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Bacterial recombination was used to insert a cre-polyA-Frt-kanamycin-Frt cassette in-frame with the start codon of Hoxa5 in the bacterial artificial chromosome RP23-20F21. Arabinose-induced Flpe expression in bacteria excised the kanamycin marker. The properly recombined BAC was linearized and used to produce transgenic animals. One founder that exhibited the expected cre expression pattern was obtained. (J:193244)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
