Bacterial recombination was used to insert a cre/ERT2-polyA-Frt-kanamycin-Frt cassette in-frame with the start codon of Mafb in the bacterial artificial chromosome RP23-33A18. Arabinose-induced Flpe expression in bacteria excised the kanamycin marker. The properly recombined BAC was linearized and used to produce transgenic animals. (J:193244)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count