A loxP site was inserted upstream of exon 5. An FRT-flanked neo cassette with a 5' loxP site was inserted downstream of exon 5. Flp-mediated recombination removed the neo cassette and left exon 5 floxed. (J:194304)
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A loxP site was inserted upstream of exon 5. An FRT-flanked neo cassette with a 5' loxP site was inserted downstream of exon 5. Flp-mediated recombination removed the neo cassette and left exon 5 floxed. (J:194304)