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Homologous recombination was employed to insert a nuclear-localized reverse tetracycline transactivator sequence and Frt-flanked PGK-neomycin cassette at the translational start site in exon 1 of Nfatc1 in a BAC. The neo cassette was removed with Flpe. The purified construct was injected into fertilized FVB 0.5 day-zygotes. Seven founder lines were obtained and bred to tetO-cre;Gt(ROSA)26Sorfslz mice, followed by doxycycline induction for 3 days starting on E7.5. Three lines (96, 100, and 122) showed expression in the endocardium and its mesenchymal descendants in the endocardial cushion. Data from #96 is provided in the original reference. (J:193198)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
