Fance^{Tg(Tyr)2364E-2a2Ove}

The Tyrosinase minigene is composed of the mouse Tyr enhancer region (623 bp), promoter region (657 bp), and 1566 bp cDNA sequence (including the stop codon). The Tyro minigene is followed by an internal ribosome entry site (IRES) ending with ATGs in all three reading frames (3xATG), an artificial splice donor sequence, and a loxP site. Using inverse PCR analysis, the lentiviral integration site was identified in intron 8 of the Fanconi anemia E gene (Fance) on chromosome 17. The 3'-LTR is linked to the (+) strand of DNA at position 28,458,210 bp [NCB137/mm9; 3'-28,458,210(+)]. (J:251511)