A loxP site was inserted in intron 4 and an FRT flanked PGK-neomycin selection cassette folled by a second loxP site was inserted in the 3' untranslated region of exon 6. The selection cassette was subsequently removed by flp expression. (J:192725)
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A loxP site was inserted in intron 4 and an FRT flanked PGK-neomycin selection cassette folled by a second loxP site was inserted in the 3' untranslated region of exon 6. The selection cassette was subsequently removed by flp expression. (J:192725)