A loxP site was inserted upstream of exon 2. An FRT-flanked self-excising neo cassette with a 5' loxP site was inserted downstream of exon 5. The neo cassette was either removed by flp-mediated recombination or spontaneously lost, leaving exons 2 through 5 floxed. (J:94077, J:192636)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count