Crb2^tm1.1Wij

A loxP site and FRT flanked neomycin selection cassette were inserted in intron 9a and a second loxP site was inserted in exon 13 in the 3' untranslated region. The selection cassette was subsequently removed from the properly targeted allele using flp recombinase. Exons 10 through the coding part of exon 13 are then flanked by loxP sites. Cre excision will remove both the transmembrane domain and the C-terminal intracellular domain which includes the FERM and the PDZ protein binding domains. (J:191149)