Tg(Slc16a1-luc)50Rttr

The murine Mct1 coding sequence was amplified from pUC18-Mct1, with the addition of a single COOH-terminal c-Myc epitope tag by PCR (primer sequences in Supplementary Table 1), and inserted between the NheI and EcoRV sites of plasmid pBI-L Tet (Clontech). This generated a plasmid with a bidirectional tetracycline-regulated promoter driving expression of both Mct1::Myc and firefly luciferase. Lines 28, 50 and 60 were generated. (J:192583)