The Flp recombinase (flp deleter), which effectively recombined the Frt sites flanking the neo gene, left behind the loxP sites flanking the exon 2. Cre-recombination removed the floxed portion. (J:192520)
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The Flp recombinase (flp deleter), which effectively recombined the Frt sites flanking the neo gene, left behind the loxP sites flanking the exon 2. Cre-recombination removed the floxed portion. (J:192520)