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The COIN module (L71 loxP site, inverted polyadenylation sequence, inverted EGFP, L66 loxP site and FRT-flanked neo cassette) was inserted after the thirtieth nucleotide of the coding exon. This insertion divides the CpG island. Flp-mediated recombination removed the neo cassette. Cre-mediated recombination restored the orientation of the EGFP to allow for expression. Western blot analysis confirmed a reduction in protein expression in heterozygous samples. (J:191980)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
