Tg(Itgax-HBEGF/OVA/EGFP)1Garbi

For generation of CD11c.DTR BAC transgenic mice, a fusion construct composed of the DNAs for the human DTR, ovalbumin fragment containing sequences encoding amino acids 140-386, and eGFP was generated and inserted at the start codon of the CD11c gene in the BAC RPCI-24-7812. The eGFP component of the fusion protein failed to give a fluorescence signal, whereas the ovalbumin protein was expressed in DC, resulting in extensive proliferation of ovalbumin-specific CD4 (OT-II) and CD8 (OT-I) cells after transfer into the CD11c.DOG mice. (J:143470)