A loxP site was inserted upstream of exon 5 and a loxP site and FRT flanked hygro cassette were inserted downstream of exon 5. Flp mediated recombination removed the hygro cassette. Cre mediated recombination removed exon 5. (J:191071)
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A loxP site was inserted upstream of exon 5 and a loxP site and FRT flanked hygro cassette were inserted downstream of exon 5. Flp mediated recombination removed the hygro cassette. Cre mediated recombination removed exon 5. (J:191071)