Pvalb^{tm3.1(dreo)Hze}

ES cells containing Pvalbtm1.1(cre)Aibs were retargeted to insert a viral 2A oligopeptide (T2A) that mediates ribosomal skipping and a mammalian-optimized D6 site-specific DNA recombinase (dreo recombinase) inserted immediately downstream of the parvalbumin translational STOP codon. Correctly targeted ES cells had (from 5' to 3') a three amino acid linker sequence, an frt3 site within Pvalb intron 3, a partial Pvalb exon 4 sequence up to (but not including) the endogenous stop codon, a T2A sequence that is in-frame with the Pvalb coding sequence, a D6 site-specific DNA recombinase optimized for mammalian cell expression (DreO recombinase), the Pvalb 3' UTR sequence from exon 4, an attB site, a PGK-hygromycin-SV40polyA cassette (with an RNA spice donor-frt5 site-RNA spice acceptor in the hygromycin gene), and an attP site. PhiC31-mediated recombination removed the hygro cassette. (J:191266, J:219930)