A targeting vector was designed to insert a loxP site upstream of exon 3 and a second loxP site downstream of exon 3, followed by a frt-flanked neomycin resistance (neo) cassette. Flp-mediated recombination remvoed the neo cassette. (J:189951)
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A targeting vector was designed to insert a loxP site upstream of exon 3 and a second loxP site downstream of exon 3, followed by a frt-flanked neomycin resistance (neo) cassette. Flp-mediated recombination remvoed the neo cassette. (J:189951)