LoxP sites were introduced approximately 250 bp 5' of Mir145 and 500 bp 3' of Mir143. A FRT-Neo-FRT cassette was also placed 3' of Mir143, just inside the floxed region. Flp-mediated recombination removed the neo cassette. (J:229235)
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LoxP sites were introduced approximately 250 bp 5' of Mir145 and 500 bp 3' of Mir143. A FRT-Neo-FRT cassette was also placed 3' of Mir143, just inside the floxed region. Flp-mediated recombination removed the neo cassette. (J:229235)