A loxP was inserted in intron 1 and a second loxP followed by an FRT flanked selection cassette were inserted in intron 2. The selection cassette was subsequently removed using flp recombinase. (J:189357)
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A loxP was inserted in intron 1 and a second loxP followed by an FRT flanked selection cassette were inserted in intron 2. The selection cassette was subsequently removed using flp recombinase. (J:189357)