One hundred and five nucleotides downstream of the first ATG codon were replaced by a cDNA for EGFP and an FRT flanked neomycin selection cassette. The neo selection cassette was subsequently removed by flp recombinase expression. (J:188987)
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One hundred and five nucleotides downstream of the first ATG codon were replaced by a cDNA for EGFP and an FRT flanked neomycin selection cassette. The neo selection cassette was subsequently removed by flp recombinase expression. (J:188987)