A loxP site was inserted upstream of exon 2 and a second loxP site followed by an FRT flanked neo cassette were inserted in intron 2. Subsequent flp recombinase expression removed the neo selection cassette. (J:190434)
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A loxP site was inserted upstream of exon 2 and a second loxP site followed by an FRT flanked neo cassette were inserted in intron 2. Subsequent flp recombinase expression removed the neo selection cassette. (J:190434)