A loxP site was inserted upstream of exon 15 and an FRT flanked neo cassette and loxP site were inserted downstream of exon 16 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:188475)
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A loxP site was inserted upstream of exon 15 and an FRT flanked neo cassette and loxP site were inserted downstream of exon 16 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:188475)