A loxP site and FRT flanked neo cassette were inserted upstream of exon 1, a 3X Flag tag was added after the initiation codon and a loxP site was inserted downstream of exon 1 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:187380)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count