A loxP site and FRT flanked neo cassette were inserted upstream of exon 4 and a loxP site was inserted downstream of exon 5 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:187380)
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A loxP site and FRT flanked neo cassette were inserted upstream of exon 4 and a loxP site was inserted downstream of exon 5 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:187380)