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DNA encoding the cre/ERT2 open reading frame, a bovine growth hormone (BGH) polyA tag, and an frt-flanked kanamycin selection cassette was inserted into the first exon of the Prrxl1 gene in BAC clone RP23-199P8 by homologous recombination in EL250 bacterial cells. Flp expression was induced to excise the kanamycin cassette. The purified BAC construct was injected into FVB/N fertilized eggs. Founder information is not available. (J:187485)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
