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Homologous recombination in bacterial cells inserted a construct with cre recombinase fused with the tamoxifen-responsive mutated estrogen receptor sequence, ERT2, into the ATG uptstream starting coding region of the tissue-nonspecific alkaline phosphatase gene (Alpl) in BAC clone RP23-400F8. The correctly targeted BAC was electroporated into fertilized eggs, producing seven founders. These were crossed to R26R reporter mice and three lines (3, 6, and 12) showed efficient cre recombination in progeny. Most subsequent experiments were performed with the line propagated from founder 12 which was more fertile than the other founders. (J:188175)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
