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Flexible Accelerated STOP Tetracycline Operator (tetO)-knockin (FAST) was used to insert a construct containing a loxP site, FRT site, neo cassette, STOP, FRT site, tetracycline operator (tetO) and loxP site was inserted upstream of the translation initiation site. Flp-mediated recombination removed the neo/STOP cassette and left the tetO floxed and intact. Autoradiography confirmed a 20% reduction in protein expression. (J:137439, J:167659)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
