A loxP site was inserted in intron 2 and an FRT flanked pgk-neo cassette and loxP site were inserted into intron 17 via homologous recombination. Recombinase mediated recombination removed exons 3-17 and the neo cassette. (J:187588)
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A loxP site was inserted in intron 2 and an FRT flanked pgk-neo cassette and loxP site were inserted into intron 17 via homologous recombination. Recombinase mediated recombination removed exons 3-17 and the neo cassette. (J:187588)