Pkhd1^tm2.1Cjwa

Pkhd1tm2Cjwa mice were crossed with Tg(Gdf9-cre)5092Coo mice to remove the LSL (loxP-STOP-loxP) cassette leaving a single loxP site in intron 2. Upon removal of the LSL, the gene is reactivated and the expressed protein carries two SV5-Pk epitope tags on its extreme N-terminus. Northern blot analysis reveals the presence of 13 kb, 9 kb, 7.7kb and 7.5kb products identical in length to products generated from the wild-type allele. (J:187735)