Il13^tm2.1Lky

A targeting vector was designed to insert a neomycin (neo) resistance cassette, and an internal ribosome entry site (IRES), followed by truncated human CD4 cDNA, between the stop codon and the 3' untranslated region. The truncated CD4 fragment(424 aa vs. 458 aa in full-length protein) lacking intracellular signaling capacity (aa 419-424 as cytoplasmic tail in truncated form vs. aa 419-458 in full-length hCD4), and also contains a point mutation resulting in an I(ATC codon) to F(TTC codon) substitution at position 43 of the protein, rendering the human CD4 marker incapable of interacting with mouse major histocompatibility complex class II (MHCII) and useful as a marker of cells expressing IL13. Cre-mediated recombination removed the neo cassette. (J:178986)