A loxP site and FRT flanked neo cassette were inserted into intron 7 and a loxP site was inserted into intron 8 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exon 8 floxed. (J:186780)
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A loxP site and FRT flanked neo cassette were inserted into intron 7 and a loxP site was inserted into intron 8 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exon 8 floxed. (J:186780)