Exon 1 was flanked by a floxed neo cassette and an additional loxP site. Cre-mediated recombination removed the neo cassette and exon 1. Western blot analysis of brush border membrane vesicles confirmed the absence of protein expression. (J:186790)
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Exon 1 was flanked by a floxed neo cassette and an additional loxP site. Cre-mediated recombination removed the neo cassette and exon 1. Western blot analysis of brush border membrane vesicles confirmed the absence of protein expression. (J:186790)