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A targeting construct containing a cre-FRT-amp-FRT cassette with an SV40 polyA sequence was used to target the Crx gene in BAC clone RP23-81H17 by recombineering methods in bacteria. The cassette was inserted after the ATG initiation codon in exon 2. The amp selection cassette was deleted in targeted clones. The purified BAC-cre construct was injected into fertilized oocytes. More than 3 lines were generated and line 60 was more extensively characterized. (J:186563)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
