A loxP site was inserted upstream of exon 1 and a floxed neo cassette was inserted immediately downstream. Properly targeted mice were crossed to cre expressing mice to excise the floxed selection cassette and exon 1. (J:186292)
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A loxP site was inserted upstream of exon 1 and a floxed neo cassette was inserted immediately downstream. Properly targeted mice were crossed to cre expressing mice to excise the floxed selection cassette and exon 1. (J:186292)