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The construct was constructed by inserting a cassette containing the flpe/ERT2 coding and polyA sequences into a Wnt1 expression-vector. 11 kb of Wnt1 genomic DNA containing critical 3' enhancer sequences were included after the FLPe/ERT2 cassette. Tandem insulator sequences flanked the complete construct. The purified transgene was injected into fertilized eggs to produce transgenic animals. 11 lines were obtained and FLPe/ERT2 mRNA expression recapitulated endogenous Wnt1 expression. Two lines showing the highest levels of FLPe/ERT2 mRNA were characterized with respect to recombinase activity. Data from line 9455 was shown in detail. (J:97320)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
