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In the Trpv6 gene a loxP site was inserted upstream of a modified exon 13 in which nucleotide substitutions (GAT to GCC) result in the amino acid substitution of alanine for aspartic acid at position 581 (p.D581A). An FRT site flanked neomycin resistance gene cassette with a 3' loxP site was inserted downstream of the last exon (15) into intron 16 of the Ephb6 gene on the opposite strand. Subsequent cre-mediated recombination removed the neo cassette, exons 13-15 of Trpv6, as well as exons 17 and 18 of Ephb6. Immunohistochemistry confirmed the absence of Trpv6 protein expression in the epididymis. (J:185996)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
