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A construct containing an eGFP gene and an inverted neo cassette was inserted in frame in exon 2 after the initiation codon via homologous recombination. RT-PCR and western blot analysis confirmed the absence of expression in the brains of homozygous mice. EGFP expression was detected by RT-PCR and live fluorescent imaging in the nervous system and liver. (J:184856)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
