Ift25^{tm1b(EUCOMM)Wtsi}

The L1L2_gt2 cassette was inserted at position 106945715 of Chromosome 4 upstream of the critical exon(s) (Build 37). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 106946668. The critical exon(s) is/are thus flanked by loxP sites. Cre mediated recombination deleted exon 3. Western blot analysis confirmed the absence of protein expression in homozygous mice. (J:184749)