Tg(Col2a1/Acan-rtTA,tetO-cre)#Vlf

A bigenic construct was generated with a 0.9kb fragment of the Col2a1 promoter, 4 tandem copies of a cartilage-specific Acan enhancer in an intron, a splice acceptor site, the reverse tetracycline transactivator coding sequence and a polyadenylation site composing the first gene. A 4 kb Trp53 intron insulator sequence separated the rtTA sequence from the second part of the construct, consisting of a heptad of tandem copies of a tetracycline-responsive element (tetO), the human cytomegalovirus promoter (hCMV), the cre recombinase coding sequence, and a polyadenylation site. No founder information for the transgene is available. Upon treatment of transgenic mice with doxycycline, the rtTA is activated and binds to tetO to induce cre expression. (J:183084)