A loxP site was inserted in the 5' untranslated region and an FRT flanked neo cassette and loxP site were inserted in the 3' untranslated region via homologous recombination. Flp mediated recombination removed the neo cassette. (J:184434)
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A loxP site was inserted in the 5' untranslated region and an FRT flanked neo cassette and loxP site were inserted in the 3' untranslated region via homologous recombination. Flp mediated recombination removed the neo cassette. (J:184434)