A targeting vector was designed to insert a single loxP site upstream of exon 3, and a loxP-flanked hygromycin resistance (hygro) cassette downstream of exon 3. Cre-mediated recombination removed the hygro cassette and left exon 3 floxed. (J:177836)
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A targeting vector was designed to insert a single loxP site upstream of exon 3, and a loxP-flanked hygromycin resistance (hygro) cassette downstream of exon 3. Cre-mediated recombination removed the hygro cassette and left exon 3 floxed. (J:177836)