A loxP site was introduced to intron 3 and FRT-neomycin-FRT-loxP sequences were added to intron 5 of the targeted gene. Flp-mediated recombination removed the neo cassette. (J:259846)
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A loxP site was introduced to intron 3 and FRT-neomycin-FRT-loxP sequences were added to intron 5 of the targeted gene. Flp-mediated recombination removed the neo cassette. (J:259846)