The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion of size 164 starting at position 17849824 and ending at position 17849988 of Chromosome 3 (Build GRCm39). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and finally an FRT site. (J:174268)