The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion of size 216 starting at position 176599829 and ending at position 176600045 of Chromosome 1 (Build GRCm39). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and finally an FRT site. (J:174268)