The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion of size 688 starting at position 194719311 and ending at position 194719999 of Chromosome 1 (Build GRCm39). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and finally an FRT site. (J:174268)