A PGK_EM7_PuDtk_bGHpA cassette replaced a genomic region of size 190 starting at position 30115899 and ending at position 30116090 of Chromosome 6 (Build GRCm39). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and finally an FRT site. (J:174268)