An FRT-flanked neo cassette with a 3' loxP site was inserted upstream of exon 11, and an additional loxP site was inserted downstream of exon 11. Flp-mediated recombination in ES cells removed the neo cassette, creating the floxed allele. (J:167711)
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An FRT-flanked neo cassette with a 3' loxP site was inserted upstream of exon 11, and an additional loxP site was inserted downstream of exon 11. Flp-mediated recombination in ES cells removed the neo cassette, creating the floxed allele. (J:167711)