A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette and a loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the neo cassette and left exons 2 through 4 floxed. (J:185263)
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A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette and a loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the neo cassette and left exons 2 through 4 floxed. (J:185263)